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KMID : 0380220070400060959
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Journal of Biochemistry and Molecular Biology
2007 Volume.40 No. 6 p.959 ~ p.965
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Isolation and Characterization of Two Korean Mistletoe Lectins
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Kang Tae-Bong
Song Seong-Kyu
Yoon Taek-Joon
Yoo Young-Choon
Lee Kwan-Hee
Her Erk
Kim Jong-Bae
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Abstract
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Two isolectins (KML-IIU and the KML-IIL) were individually isolated from the previously reported Korean mistletoe lectin, KML-C, by using an immunoaffinity column. Molecular weights of the KML-IIU and the KML-IIL were 64 kDa and 60 kDa respectively. Both of the lectins were composed of heterogeneous A and B subunits linked with a disulfide bond, and showed the same carbohydrate-binding specificities for Gal and GalNAc. However, they are different not only in biophysical properties (glycosylation and amino acid compositions) but also bioactivities (cell killing and cytokine induction). The KML-IIL showed 17-145 times stronger in cytotoxicities to various human and mouse cancer cell lines than the KML-IIU. The KML-IIL also induced TNF-&agr; secretion from mouse peritoneal macrophages 4.5 times better than the KML-IIU. The results demonstrated isolectins in Korean mistletoe were varied in bioactivities and the KML-IIL may be developed as an anti-cancer agent.
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KEYWORD
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Cytotoxic effect, Glycosylation, Isolectin, Mistletoe, TNF-&agr,
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